Lactobacillus casei asp23 gene contributes to gentamycin resistance via regulating specific membrane-associated proteins

Adaptation of Lactobacillus casei Zhang to Gentamycin Involves an Alkaline Shock Protein

Lactobacillus (L. casei) Zhang is a koumiss-originated probiotic strain, which was used as a model in a long-term antibiotics-driven evolution experiment to reveal bacterial evolutionary dynamics; and we isolated gentamycin-resistant L. casei Zhang descendents. To decipher the gentamycin resistance mechanism, here we cultivated the parental L. casei Zhang and its descendent cells in an antibiot...

Membrane-bound ATPase contributes to hop resistance of Lactobacillus brevis.

The activity of the membrane-bound H+-ATPase of the beer spoilage bacterium Lactobacillus brevis ABBC45 increased upon adaptation to bacteriostatic hop compounds. The ATPase activity was optimal around pH 5.6 and increased up to fourfold when L. brevis was exposed to 666 microM hop compounds. The extent of activation depended on the concentration of hop compounds and was maximal at the highest ...

“Drug resistance associated membrane proteins”

Study of drug resistance began in the late 1940’s, and recognition that altered membrane transport of drug was often related to cellular drug resistance followed approximately 20 years later. Identification and isolation of specific membrane proteins that influence this altered drug transport began in the 1970’s and is a major ongoing endeavor to this day. In this article, we refer to such prot...

D-Alanine: membrane acceptor ligase from Lactobacillus casei.

The enzymatic incorporation of D-alanine into membranes of Lacfobacillus casei (ATCC 7469) is described. The activity is dependent on ATP, supernatant fraction and membrane fragments; it is enhanced by the addition of Mg2+. The incorporation is insensitive to ribonuclease. The product of this reaction is characterized as a hydroxylamine (1 M, pH 7.0, 37”) labile ester that is not extracted into...

The Membrane Bound ATPase Contributes to Hop Resistance of Lactobacillus brevis